NT5E sirna

NT5E siRNA (Human)

Cat. Num. AAA8215713

• Gene Names: NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NT5E; NT5E siRNA (Human); NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD antigen CD73; NT5E sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NT5E siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 524

• Applicable Applications for NT5E sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NT5E gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NT5E sirna

siRNA to inhibit NT5E expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 325651886
• NCBI GeneID: 4907
• NCBI Accession #: NP_001191742.1
• NCBI GenBank Nucleotide #: NM_001204813.1
• UniProt Accession #: P21589; O75520; Q5W116; B3KQI8
• Molecular Weight: 57,949 Da
• NCBI Official Full Name: 5'-nucleotidase isoform 2 preproprotein
• NCBI Official Synonym Full Names: 5'-nucleotidase, ecto (CD73)
• NCBI Official Symbol: NT5E
• NCBI Official Synonym Symbols: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: NT5E
• UniProt Synonym Gene Names: NT5; NTE; 5'-NT
• UniProt Entry Name: 5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Membrane protein, GPI anchor; EC 3.1.3.5; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries