NSF sirna

NSF siRNA (Human)

Cat. Num. AAA8227267

• Gene Names: NSF; SKD2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NSF; NSF siRNA (Human); Vesicle-fusing ATPase; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF; NSF sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NSF siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 644

• Applicable Applications for NSF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NSF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NSF sirna

siRNA to inhibit NSF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156564401
• NCBI GeneID: 4905
• NCBI Accession #: NP_006169.2
• NCBI GenBank Nucleotide #: NM_006178.3
• UniProt Accession #: P46459; Q8N6D7; Q9UKZ2; A8K2D9; B4DFA2
• Molecular Weight: 71,584 Da
• NCBI Official Full Name: vesicle-fusing ATPase
• NCBI Official Synonym Full Names: N-ethylmaleimide-sensitive factor
• NCBI Official Symbol: NSF
• NCBI Official Synonym Symbols: SKD2
• NCBI Protein Information: vesicle-fusing ATPase
• UniProt Protein Name: Vesicle-fusing ATPase
• Protein Family: NSFL1 cofactor
• UniProt Gene Name: NSF
• UniProt Synonym Gene Names: NEM-sensitive fusion protein
• UniProt Entry Name: NSF_HUMAN

Uniprot Description

NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

Protein type: EC 3.6.4.6; Adaptor/scaffold; Autophagy; Protease; Vesicle

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: Golgi stack; lysosomal membrane; cytoplasm; postsynaptic density; plasma membrane; dendritic shaft; cytosol

Molecular Function: protein binding; metal ion binding; ATPase activity; protein complex binding; syntaxin-1 binding; ATPase activity, coupled; protein kinase binding; Rab GTPase binding; ATP binding; PDZ domain binding

Biological Process: vesicle-mediated transport; regulation of exocytosis; intra-Golgi vesicle-mediated transport; synaptic transmission; exocytosis; metabolic process; Golgi to plasma membrane protein transport; Golgi vesicle docking; positive regulation of receptor recycling; potassium ion transport; plasma membrane fusion