NFYB sirna

NFYB siRNA (Human)

Cat. Num. AAA8209651

• Gene Names: NFYB; HAP3; CBF-A; CBF-B; NF-YB
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NFYB; NFYB siRNA (Human); HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB; NFYB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NFYB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 207

• Applicable Applications for NFYB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NFYB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NFYB sirna

siRNA to inhibit NFYB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5453780
• NCBI GeneID: 4801
• NCBI Accession #: NP_006157.1
• NCBI GenBank Nucleotide #: NM_006166.3
• UniProt Accession #: P25208; Q96IY8; A8K7B9
• Molecular Weight: 22,831 Da
• NCBI Official Full Name: nuclear transcription factor Y subunit beta
• NCBI Official Synonym Full Names: nuclear transcription factor Y, beta
• NCBI Official Symbol: NFYB
• NCBI Official Synonym Symbols: HAP3; CBF-A; CBF-B; NF-YB
• NCBI Protein Information: nuclear transcription factor Y subunit beta
• UniProt Protein Name: Nuclear transcription factor Y subunit beta
• Protein Family: Nuclear transcription factor
• UniProt Gene Name: NFYB
• UniProt Synonym Gene Names: HAP3; NF-YB
• UniProt Entry Name: NFYB_HUMAN

NCBI Description

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]

Uniprot Description

NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; protein complex binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process