NFKBIZ sirna

NFKBIZ siRNA (Human)

Cat. Num. AAA8209596

• Gene Names: NFKBIZ; IKBZ; INAP; MAIL
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NFKBIZ; NFKBIZ siRNA (Human); IKBZ; INAP; MAIL; NF-kappa-B inhibitor zeta; I-kappa-B-zeta; IkB-zeta; IkappaBzeta; IL-1 inducible nuclear ankyrin-repeat protein; Molecule possessing ankyrin repeats induced by lipopolysaccharide; NFKBIZ sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NFKBIZ siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 618

• Applicable Applications for NFKBIZ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NFKBIZ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NFKBIZ sirna

siRNA to inhibit NFKBIZ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 53832024
• NCBI GeneID: 64332
• NCBI Accession #: NP_001005474.1
• NCBI GenBank Nucleotide #: NM_001005474.2
• UniProt Accession #: Q9BYH8; Q8IUL4; Q8NAZ8; B3KNR2; D3DN54
• Molecular Weight: 64,298 Da
• NCBI Official Full Name: NF-kappa-B inhibitor zeta isoform b
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
• NCBI Official Symbol: NFKBIZ
• NCBI Official Synonym Symbols: IKBZ; INAP; MAIL
• NCBI Protein Information: NF-kappa-B inhibitor zeta
• UniProt Protein Name: NF-kappa-B inhibitor zeta
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIZ
• UniProt Synonym Gene Names: IKBZ; INAP; MAIL; IkB-zeta; IkappaBzeta; INAP; MAIL
• UniProt Entry Name: IKBZ_HUMAN

NCBI Description

This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IkB-zeta: Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF- kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis. Interacts with NFKB1/p50. Interacts with RELA. By TNF, IL1/interleukin-1 and bacterial lipopolysaccharides (LPS). Expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 3p12-q12

Cellular Component: nucleus

Molecular Function: transcription cofactor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; inflammatory response