Rabbit anti-Human RPS14 Polyclonal Antibody | anti-RPS14 antibody

RPS14, CT (RPS14, 40S ribosomal protein S14) (Biotin)

Cat. Num. AAA6343734

• Gene Names: RPS14; S14; EMTB
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS14; Polyclonal Antibody; CT (RPS14; 40S ribosomal protein S14) (Biotin); 40S ribosomal protein S14; anti-RPS14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RPS14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPS14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-147 amino acids from the C-terminal region of human RPS14.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPS14 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RPS14 antibody detected the RPS14 protein (arrow).)

Related Product Information for anti-RPS14 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-RPS14 antibody

Antibodies; Ribosomal Proteins

References

Oliva, E.N., et al. Eur. J. Haematol. 85(3):231-235(2010). Borze, I., et al. Cancer Genet. Cytogenet. 197(2):166-173(2010). Quarello, P., et al. Haematologica 95(2):206-213(2010). Valencia, A., et al. Blood 112 (3), 918 (2008): Ebert, B.L., et al. Nature 451(7176):335-339(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 68160915
• NCBI GeneID: 6208
• NCBI Accession #: NP_001020241.1; NP_005608.1
• NCBI GenBank Nucleotide #: NM_001025070.1
• UniProt Accession #: P62263; P06366; Q5BJI0; B2R5G5; D3DQG5
• Molecular Weight: 16,273 Da
• NCBI Official Full Name: 40S ribosomal protein S14
• NCBI Official Synonym Full Names: ribosomal protein S14
• NCBI Official Symbol: RPS14
• NCBI Official Synonym Symbols: S14; EMTB
• NCBI Protein Information: 40S ribosomal protein S14; emetine resistance
• UniProt Protein Name: 40S ribosomal protein S14
• Protein Family: Ribosomal protein
• UniProt Gene Name: RPS14
• UniProt Entry Name: RS14_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS14: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: RNA-binding; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: focal adhesion; membrane; mitochondrion; cytoplasm; nucleolus; cytosol

Molecular Function: structural constituent of ribosome; RNA binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; translational termination; maturation of SSU-rRNA; regulation of translation; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; erythrocyte differentiation; gene expression; viral transcription

Disease: Chromosome 5q Deletion Syndrome