Rabbit NFKBIZ Polyclonal Antibody | anti-NFKBIZ antibody

Anti-NFKBIZ Antibody

Cat. Num. AAA416207

• Gene Names: NFKBIZ; IKBZ; INAP; MAIL
• Reactivity: Human. Predicted: Mouse, Rat.
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: NFKBIZ; Polyclonal Antibody; Anti-NFKBIZ Antibody; anti-NFKBIZ antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat.
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 618

• Applicable Applications for anti-NFKBIZ antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:1,000-1:2,000; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal NFKBIZ (1) antibody was raised against a peptide from C terminal residues of human NFKBIZ protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-NFKBIZ antibody

This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

References

(1) Lorenz,J., Zahlten,J., Pollok,I. et al. Legionella pneumophila-induced IkappaBzeta-dependent expression of interleukin-6 in lung epithelium. Eur. Respir. J. 37 (3), 648-657 (2011).

NCBI and Uniprot Product Information

• NCBI GI #: 53832024
• NCBI GeneID: 64332
• NCBI Accession #: NP_001005474.1
• NCBI GenBank Nucleotide #: NM_001005474.2
• Molecular Weight: 64,298 Da
• NCBI Official Full Name: NF-kappa-B inhibitor zeta isoform b
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
• NCBI Official Symbol: NFKBIZ
• NCBI Official Synonym Symbols: IKBZ; INAP; MAIL
• NCBI Protein Information: NF-kappa-B inhibitor zeta; I-kappa-B-zeta; IL-1 inducible nuclear ankyrin-repeat protein; Ikappa B-zeta variant 3; IkappaB-zeta; ikB-zeta; ikappaBzeta; molecule possessing ankyrin repeats induced by lipopolysaccharide
• UniProt Protein Name: NF-kappa-B inhibitor zeta
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIZ
• UniProt Entry Name: IKBZ_HUMAN

NCBI Description

This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IkB-zeta: Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF- kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis. Interacts with NFKB1/p50. Interacts with RELA. By TNF, IL1/interleukin-1 and bacterial lipopolysaccharides (LPS). Expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 3p12-q12

Cellular Component: nucleus

Molecular Function: transcription cofactor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; inflammatory response