NDUFAF5 sirna

NDUFAF5 siRNA (Human)

Cat. Num. AAA8211325

• Gene Names: NDUFAF5; C20orf7; dJ842G6.1; bA526K24.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NDUFAF5; NDUFAF5 siRNA (Human); C20orf7; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5; Probable methyltransferase C20orf7 mitochondrial; NDUFAF5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NDUFAF5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 317

• Applicable Applications for NDUFAF5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NDUFAF5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NDUFAF5 sirna

siRNA to inhibit NDUFAF5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 86792933
• NCBI GeneID: 79133
• NCBI Accession #: NP_001034464.1
• NCBI GenBank Nucleotide #: NM_001039375.2
• UniProt Accession #: Q5TEU4; Q6GPH3; Q9H6F4; A8K166
• Molecular Weight: 36,076 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) complex I, assembly factor 5
• NCBI Official Symbol: NDUFAF5
• NCBI Official Synonym Symbols: C20orf7; dJ842G6.1; bA526K24.2
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5
• Protein Family: Arginine-hydroxylase
• UniProt Gene Name: NDUFAF5
• UniProt Synonym Gene Names: C20orf7
• UniProt Entry Name: NDUF5_HUMAN

NCBI Description

The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

NDUFAF5: Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages. May have methyltransferase activity. Defects in NDUFAF5 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Defects in NDUFAF5 are a cause of Leigh syndrome (LS). An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia. Belongs to the methyltransferase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Mitochondrial; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 20p12.1

Molecular Function: methyltransferase activity

Biological Process: methylation; mitochondrial respiratory chain complex I assembly

Disease: Mitochondrial Complex I Deficiency