N4BP2 sirna

N4BP2 siRNA (Human)

Cat. Num. AAA8224929

• Gene Names: N4BP2; B3BP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: N4BP2; N4BP2 siRNA (Human); B3BP; KIAA1413; NEDD4-binding protein 2; BCL-3-binding protein; N4BP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: N4BP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1770

• Applicable Applications for N4BP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human N4BP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for N4BP2 sirna

siRNA to inhibit N4BP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31742492
• NCBI GeneID: 55728
• NCBI Accession #: NP_060647.2
• NCBI GenBank Nucleotide #: NM_018177.4
• UniProt Accession #: Q86UW6; Q9NVK2; Q9P2D4; A0AVR3
• Molecular Weight: 196,828 Da
• NCBI Official Full Name: NEDD4-binding protein 2
• NCBI Official Synonym Full Names: NEDD4 binding protein 2
• NCBI Official Symbol: N4BP2
• NCBI Official Synonym Symbols: B3BP
• NCBI Protein Information: NEDD4-binding protein 2
• UniProt Protein Name: NEDD4-binding protein 2
• Protein Family: NEDD4-binding protein
• UniProt Gene Name: N4BP2
• UniProt Synonym Gene Names: B3BP; KIAA1413; N4BP2
• UniProt Entry Name: N4BP2_HUMAN

NCBI Description

This gene encodes a protein containing a polynucleotide kinase domain (PNK) near the N-terminal region, and a Small MutS Related (Smr) domain near the C-terminal region. The encoded protein can bind to both B-cell leukemia/lymphoma 3 (BCL-3) and neural precursor cell expressed, developmentally downregulated 4, (Nedd4) proteins. This protein binds and hydrolyzes ATP, may function as a 5'-polynucleotide kinase, and has the capacity to be a ubiquitylation substrate. This protein may play a role in transcription-coupled DNA repair or genetic recombination. [provided by RefSeq, Jul 2008]

Uniprot Description

Nedd4-BP2: Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA repair or recombination. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; Hydrolase; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: cytosol

Molecular Function: protein binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; endonuclease activity; ATP binding

Biological Process: phosphorylation