MAFA sirna

MAFA siRNA (Human)

Cat. Num. AAA8228550

• Gene Names: MAFA; hMafA; RIPE3b1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAFA; MAFA siRNA (Human); Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; Transcription factor RIPE3b1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAFA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 353

• Applicable Applications for MAFA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAFA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAFA sirna

siRNA to inhibit MAFA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 71274111
• NCBI GeneID: 389692
• NCBI Accession #: NP_963883.2
• NCBI GenBank Nucleotide #: NM_201589.3
• UniProt Accession #: Q8NHW3
• Molecular Weight: 36,982 Da
• NCBI Official Full Name: transcription factor MafA
• NCBI Official Synonym Full Names: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
• NCBI Official Symbol: MAFA
• NCBI Official Synonym Symbols: hMafA; RIPE3b1
• NCBI Protein Information: transcription factor MafA
• UniProt Protein Name: Transcription factor MafA
• Protein Family: Transcription factor
• UniProt Gene Name: MAFA
• UniProt Entry Name: MAFA_HUMAN

NCBI Description

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008]

Uniprot Description

MAFA: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1. Up-regulated by glucose. Belongs to the bZIP family. Maf subfamily.

Protein type: Tumor suppressor; Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; insulin secretion; response to glucose stimulus; positive regulation of transcription from RNA polymerase II promoter; nitric oxide mediated signal transduction