Typical Testing Data/Standard Curve (for reference only)
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Monkey Transcription factor MafA (MAFA) ELISA Kit | MAFA elisa kit
Monkey Transcription factor MafA (MAFA) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: MAFA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MAFA antibody and an MAFA-HRP conjugate. The assay sample and buffer are incubated together with MAFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAFA concentration since MAFA from samples and MAFA-HRP conjugate compete for the anti-MAFA antibody binding site. Since the number of sites is limited, as more sites are occupied by MAFA from the sample, fewer sites are left to bind MAFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAFA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008]
Uniprot Description
MAFA: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1. Up-regulated by glucose. Belongs to the bZIP family. Maf subfamily.
Protein type: Oncoprotein; DNA-binding; Tumor suppressor; Transcription factor
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: nucleus
Molecular Function: protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; insulin secretion; response to glucose stimulus; positive regulation of transcription from RNA polymerase II promoter; nitric oxide mediated signal transduction
Research Articles on MAFA
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Product Notes
The Monkey MAFA mafa (Catalog #AAA7232000) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7232000 ELISA Kit recognizes Monkey MAFA. It is sometimes possible for the material contained within the vial of "Transcription factor MafA (MAFA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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