L3MBTL1 sirna

L3MBTL1 siRNA (Human)

Cat. Num. AAA8231843

• Gene Names: L3MBTL1; L3MBTL; ZC2HC3; H-L(3)MBT; dJ138B7.3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: L3MBTL1; L3MBTL1 siRNA (Human); KIAA0681; L3MBT; L3MBTL; Lethal(3)malignant brain tumor-like protein 1; H-l(3)mbt; H-l(3)mbt protein; L(3)mbt-like; L(3)mbt protein homolog; L3MBTL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: L3MBTL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 772

• Applicable Applications for L3MBTL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human L3MBTL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for L3MBTL1 sirna

siRNA to inhibit L3MBTL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 117938328
• NCBI GeneID: 26013
• NCBI Accession #: NP_056293.4
• NCBI GenBank Nucleotide #: NM_015478.6
• UniProt Accession #: Q9Y468; Q5H8Y8; Q5H8Y9; Q8IUV7; Q9H1E6; Q9H1G5; Q9UG06; Q9UJB9; Q9Y4C9; B4DRC9; E1P5W7
• Molecular Weight: 92,297 Da
• NCBI Official Full Name: lethal(3)malignant brain tumor-like protein 1 isoform I
• NCBI Official Synonym Full Names: l(3)mbt-like 1 (Drosophila)
• NCBI Official Symbol: L3MBTL1
• NCBI Official Synonym Symbols: L3MBTL; ZC2HC3; H-L(3)MBT; dJ138B7.3
• NCBI Protein Information: lethal(3)malignant brain tumor-like protein 1
• UniProt Protein Name: Lethal(3)malignant brain tumor-like protein 1
• Protein Family: Lethal(3)malignant brain tumor-like protein
• UniProt Gene Name: L3MBTL1
• UniProt Synonym Gene Names: KIAA0681; L3MBT; L3MBTL; H-l(3)mbt; H-l(3)mbt protein; L(3)mbt-like
• UniProt Entry Name: LMBL1_HUMAN

NCBI Description

This gene represents a polycomb group gene. The encoded protein functions to regulate gene activity, likely via chromatin modification. The encoded protein may also be necessary for mitosis. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

Uniprot Description

L3MBTL: Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post- translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Histone-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; nucleolus; plasma membrane; condensed chromosome; chromatin; nucleus

Molecular Function: identical protein binding; protein binding; nucleosomal histone binding; histone binding; zinc ion binding; nucleosome binding; SAM domain binding; chromatin binding; transcription factor activity; methylated histone residue binding

Biological Process: transcription, DNA-dependent; regulation of cell cycle; regulation of megakaryocyte differentiation; regulation of mitosis; hemopoiesis; chromatin modification; negative regulation of transcription, DNA-dependent