KLF7 sirna

KLF7 siRNA (Human)

Cat. Num. AAA8207418

• Gene Names: KLF7; UKLF
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KLF7; KLF7 siRNA (Human); UKLF; Krueppel-like factor 7; Ubiquitous krueppel-like factor; KLF7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KLF7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 87

• Applicable Applications for KLF7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KLF7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KLF7 sirna

siRNA to inhibit KLF7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 401709918
• NCBI GeneID: 8609
• NCBI Accession #: NP_001257871.1
• NCBI GenBank Nucleotide #: NM_001270942.1
• UniProt Accession #: O75840; Q7Z3H8; Q96E51; B2RB03; B7Z4F7; C9JF04; E7EWH1; L0R4P2
• Molecular Weight: 9,295 Da
• NCBI Official Full Name: Krueppel-like factor 7 isoform 2
• NCBI Official Synonym Full Names: Kruppel-like factor 7 (ubiquitous)
• NCBI Official Symbol: KLF7
• NCBI Official Synonym Symbols: UKLF
• NCBI Protein Information: Krueppel-like factor 7
• UniProt Protein Name: Krueppel-like factor 7
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF7
• UniProt Synonym Gene Names: UKLF
• UniProt Entry Name: KLF7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Kruppel-like transcriptional regulator family. Members in this family regulate cell proliferation, differentiation and survival and contain three C2H2 zinc fingers at the C-terminus that mediate binding to GC-rich sites. This protein may contribute to the progression of type 2 diabetes by inhibiting insulin expression and secretion in pancreatic beta-cells and by deregulating adipocytokine secretion in adipocytes. A pseudogene of this gene is located on the long arm of chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

KLF7: Transcriptional activator. Binds in vitro to the CACCC motif of the beta-globin promoter and to the SP1 recognition sequence. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: nucleus

Molecular Function: DNA binding; zinc ion binding; transcription coactivator activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; axon guidance; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; dendrite morphogenesis