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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using KLF7 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Rabbit KLF7 Polyclonal Antibody | anti-KLF7 antibody

KLF7 Polyclonal Antibody

Gene Names
KLF7; UKLF
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Affinity purification
Synonyms
KLF7; Polyclonal Antibody; KLF7 Polyclonal Antibody; anti-KLF7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
230
Applicable Applications for anti-KLF7 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000, IP: 1:20 - 1:50
Immunogen
Recombinant protein of human KLF7
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using KLF7 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using KLF7 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Product Categories/Family for anti-KLF7 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 33kDa
NCBI Official Full Name
Krueppel-like factor 7 isoform 2
NCBI Official Synonym Full Names
Kruppel-like factor 7 (ubiquitous)
NCBI Official Symbol
KLF7
NCBI Official Synonym Symbols
UKLF
NCBI Protein Information
Krueppel-like factor 7
UniProt Protein Name
Krueppel-like factor 7
Protein Family
UniProt Gene Name
KLF7
UniProt Synonym Gene Names
UKLF
UniProt Entry Name
KLF7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Kruppel-like transcriptional regulator family. Members in this family regulate cell proliferation, differentiation and survival and contain three C2H2 zinc fingers at the C-terminus that mediate binding to GC-rich sites. This protein may contribute to the progression of type 2 diabetes by inhibiting insulin expression and secretion in pancreatic beta-cells and by deregulating adipocytokine secretion in adipocytes. A pseudogene of this gene is located on the long arm of chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

KLF7: Transcriptional activator. Binds in vitro to the CACCC motif of the beta-globin promoter and to the SP1 recognition sequence. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: nucleus

Molecular Function: DNA binding; transcription coactivator activity; transcription factor activity; zinc ion binding

Biological Process: axon guidance; dendrite morphogenesis; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Research Articles on KLF7

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Product Notes

The KLF7 klf7 (Catalog #AAA9129225) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLF7 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KLF7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500 - 1:2000, IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the KLF7 klf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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