KHK sirna

KHK siRNA (Human)

Cat. Num. AAA8219276

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KHK; KHK siRNA (Human); Ketohexokinase; Hepatic fructokinase; KHK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KHK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 298

• Applicable Applications for KHK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KHK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KHK sirna

siRNA to inhibit KHK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4557693
• NCBI GeneID: 3795
• NCBI Accession #: NP_000212.1
• NCBI GenBank Nucleotide #: NM_000221.2
• UniProt Accession #: P50053; Q6IBK2; Q99532; Q9BRJ3; Q9UMN1
• Molecular Weight: 32,730 Da
• NCBI Official Full Name: ketohexokinase isoform a
• NCBI Official Synonym Full Names: ketohexokinase (fructokinase)
• NCBI Official Symbol: KHK
• NCBI Protein Information: ketohexokinase
• UniProt Protein Name: Ketohexokinase
• Protein Family: Ketohexokinase
• UniProt Gene Name: KHK
• UniProt Entry Name: KHK_HUMAN

NCBI Description

This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

KHK iso2: Defects in KHK are the cause of fructosuria (FRUCT). Benign defect of intermediary metabolism. Belongs to the carbohydrate kinase PfkB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.3; Transferase; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: ketohexokinase activity; ATP binding

Biological Process: carbohydrate metabolic process; carbohydrate phosphorylation; pathogenesis; fructose catabolic process

Disease: Fructosuria, Essential