KCNMB4 sirna

KCNMB4 siRNA (Human)

Cat. Num. AAA8201372

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNMB4; KCNMB4 siRNA (Human); Calcium-activated potassium channel subunit beta-4; BK channel subunit beta-4; BKbeta4; Hbeta4; Calcium-activated potassium channel. subfamily M subunit beta-4; Charybdotoxin receptor subunit beta-4; K(VCA)beta-4; Maxi K channel subunit beta-4; Slo-beta-4; KCNMB4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KCNMB4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 210

• Applicable Applications for KCNMB4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNMB4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNMB4 sirna

siRNA to inhibit KCNMB4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 26051275
• NCBI GeneID: 27345
• NCBI Accession #: NP_055320.4
• NCBI GenBank Nucleotide #: NM_014505.5
• UniProt Accession #: Q86W47; Q8IVR3; Q9NPA4; Q9P0G5
• Molecular Weight: 23,949 Da
• NCBI Official Full Name: calcium-activated potassium channel subunit beta-4
• NCBI Official Synonym Full Names: potassium channel subfamily M regulatory beta subunit 4
• NCBI Official Symbol: KCNMB4
• NCBI Protein Information: calcium-activated potassium channel subunit beta-4
• UniProt Protein Name: Calcium-activated potassium channel subunit beta-4
• Protein Family: Calcium-activated potassium channel
• UniProt Gene Name: KCNMB4
• UniProt Synonym Gene Names: BKbeta4; Hbeta4
• UniProt Entry Name: KCMB4_HUMAN

NCBI Description

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which slows activation kinetics, leads to steeper calcium sensitivity, and shifts the voltage range of current activation to more negative potentials than does the beta 1 subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

KCNMB4: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. May decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. Makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations. Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB4 subfamily.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q

Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; calcium-activated potassium channel activity; potassium channel regulator activity

Biological Process: regulation of action potential; synaptic transmission; regulation of vasoconstriction; regulation of neurotransmitter secretion; generation of action potential; detection of calcium ion; blood coagulation; potassium ion transport