KCNJ10 sirna

KCNJ10 siRNA (Human)

Cat. Num. AAA827714

• Gene Names: KCNJ10; KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNJ10; KCNJ10 siRNA (Human); ATP-sensitive inward rectifier potassium channel 10; ATP-dependent inwardly rectifying potassium channel Kir4.1; Inward rectifier K(+) channel Kir1.2; Potassium channel. inwardly rectifying subfamily J member 10; KCNJ10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KCNJ10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 379

• Applicable Applications for KCNJ10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNJ10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNJ10 sirna

siRNA to inhibit KCNJ10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 25121966
• NCBI GeneID: 3766
• NCBI Accession #: NP_002232.2
• NCBI GenBank Nucleotide #: NM_002241.4
• UniProt Accession #: P78508; Q5VUT9; Q8N4I7; Q92808; A3KME7
• Molecular Weight: 42,508 Da
• NCBI Official Full Name: ATP-sensitive inward rectifier potassium channel 10
• NCBI Official Synonym Full Names: potassium channel, inwardly rectifying subfamily J, member 10
• NCBI Official Symbol: KCNJ10
• NCBI Official Synonym Symbols: KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN
• NCBI Protein Information: ATP-sensitive inward rectifier potassium channel 10
• UniProt Protein Name: ATP-sensitive inward rectifier potassium channel 10
• Protein Family: ATP-sensitive inward rectifier potassium channel
• UniProt Gene Name: KCNJ10
• UniProt Entry Name: KCJ10_HUMAN

NCBI Description

This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]

Uniprot Description

Kir4.1: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Defects in KCNJ10 are the cause of seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance (SESAMES). A complex disorder characterized by generalized seizures with onset in infancy, delayed psychomotor development, ataxia, sensorineural hearing loss, hypokalemia, metabolic alkalosis, and hypomagnesemia. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Channel, potassium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: microvillus; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; ATP-activated inward rectifier potassium channel activity; ATP binding; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; myelination in the central nervous system; membrane hyperpolarization; response to glucocorticoid stimulus; glutamate uptake during transmission of nerve impulse; response to blue light; response to mineralocorticoid stimulus; adult walking behavior; protein homotetramerization; synaptic transmission; potassium ion import; visual perception; optic nerve development; regulation of resting membrane potential; regulation of sensory perception of pain; inflammatory response; potassium ion homeostasis; potassium ion transport

Disease: Pendred Syndrome; Seizures, Sensorineural Deafness, Ataxia, Mental Retardation, And Electrolyte Imbalance; Deafness, Autosomal Recessive 4, With Enlarged Vestibular Aqueduct