KCNE1L sirna

KCNE1L siRNA (Human)

Cat. Num. AAA829993

• Gene Names: KCNE5; KCNE1L
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNE1L; KCNE1L siRNA (Human); AMMECR2; Potassium voltage-gated channel subfamily E member 1-like protein; AMME syndrome candidate gene 2 protein; KCNE1L sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KCNE1L siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 142

• Applicable Applications for KCNE1L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNE1L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNE1L sirna

siRNA to inhibit KCNE1L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6912442
• NCBI GeneID: 23630
• NCBI Accession #: NP_036414.1
• NCBI GenBank Nucleotide #: NM_012282.2
• UniProt Accession #: Q9UJ90; Q5JWV7
• Molecular Weight: 14,993 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily E regulatory beta subunit 5
• NCBI Official Synonym Full Names: potassium channel, voltage gated subfamily E regulatory beta subunit 5
• NCBI Official Symbol: KCNE5
• NCBI Official Synonym Symbols: KCNE1L
• NCBI Protein Information: potassium voltage-gated channel subfamily E regulatory beta subunit 5
• UniProt Protein Name: Potassium voltage-gated channel subfamily E regulatory beta subunit 5
• UniProt Gene Name: KCNE5
• UniProt Entry Name: KCNE5_HUMAN

NCBI Description

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a membrane protein which has sequence similarity to the KCNE1 gene product, a member of the potassium channel, voltage-gated, isk-related subfamily. This intronless gene is deleted in AMME contiguous gene syndrome and may be involved in the cardiac and neurologic abnormalities found in the AMME contiguous gene syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

KCNE1L: Defects in KCNE1L are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR). A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. Belongs to the potassium channel KCNE family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: voltage-gated potassium channel complex

Molecular Function: voltage-gated potassium channel activity; potassium channel regulator activity

Biological Process: regulation of heart contraction; cardiac muscle contraction