IQSEC2 sirna

IQSEC2 siRNA (Human)

Cat. Num. AAA8218712

• Gene Names: IQSEC2; MRX1; BRAG1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IQSEC2; IQSEC2 siRNA (Human); KIAA0522; IQ motif and SEC7 domain-containing protein 2; IQSEC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IQSEC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1488

• Applicable Applications for IQSEC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IQSEC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IQSEC2 sirna

siRNA to inhibit IQSEC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 162138911
• NCBI GeneID: 23096
• NCBI Accession #: NP_001104595.1
• NCBI GenBank Nucleotide #: NM_001111125.2
• UniProt Accession #: Q5JU85; O60275; Q5JUX1; B3KT97; C7SDG1
• Molecular Weight: 105,100 Da
• NCBI Official Full Name: IQ motif and SEC7 domain-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: IQ motif and Sec7 domain 2
• NCBI Official Symbol: IQSEC2
• NCBI Official Synonym Symbols: MRX1; BRAG1
• NCBI Protein Information: IQ motif and SEC7 domain-containing protein 2
• UniProt Protein Name: IQ motif and SEC7 domain-containing protein 2
• Protein Family: IQ motif and SEC7 domain-containing protein
• UniProt Gene Name: IQSEC2
• UniProt Synonym Gene Names: KIAA0522
• UniProt Entry Name: IQEC2_HUMAN

NCBI Description

This gene encodes a guanine nucleotide exchange factor for the ARF family of small GTP-binding proteins. The encoded protein is a component of the postsynaptic density at excitatory synapses, and may play a critical role in cytoskeletal and synaptic organization through the activation of selected ARF substrates including ARF1 and ARF6. Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

IQSEC2: Defects in IQSEC2 are the cause of mental retardation X- linked type 1 (MRX1). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation. Belongs to the BRAG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, ARF

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: trans-Golgi network; nucleus; cell junction

Molecular Function: ARF guanyl-nucleotide exchange factor activity

Biological Process: vesicle-mediated transport; regulation of ARF protein signal transduction; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity

Disease: Mental Retardation, X-linked 1