Rabbit IQSEC2 Polyclonal Antibody | anti-IQSEC2 antibody

IQSEC2 Antibody

Cat. Num. AAA153419

• Gene Names: IQSEC2; MRX1; BRAG1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: IQSEC2 antibody is affinity chromatography purified via peptide column.
• Synonyms: IQSEC2; Polyclonal Antibody; IQSEC2 Antibody; IQ motif and Sec7 domain 2; Brefeldin-resistant Arf-GEF 1 protein; BRAG1; MRX1; anti-IQSEC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IQSEC2 antibody is human, mouse and rat reactive. At least two isoforms of IQSEC2 are known to exist; this antibody will only detect the larger isoform. IQSEC2 antibody is predicted to not cross-react with IQSEC1.
• Purity/Purification: IQSEC2 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1488

• Applicable Applications for anti-IQSEC2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IQSEC2 antibody can be used for detection of IQSEC2 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: IQSEC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IQSEC2.
• Buffer: IQSEC2 antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IQSEC2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of IQSEC2 in SK-N-SH cell lysate with IQSEC2 antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of IQSEC2 in mouse brain tissue with IQSEC2 antibody at 5 μg/ml.)

Related Product Information for anti-IQSEC2 antibody

ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. Similar to the related protein IQSEC1, IQSEC2 is a guanine nucleotide exchange protein for ARF1 and ARF6 that belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1,2). IQSEC2 is a major component of the postsynaptic density (PSD) and colocalizes with PSD-95. It has been suggested to modulate the functions of the ARF proteins (1). Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation (3).

NCBI and Uniprot Product Information

• NCBI GI #: 162138911
• NCBI GeneID: 23096
• NCBI Accession #: NP_001104595
• NCBI GenBank Nucleotide #: NM_001111125.2
• UniProt Accession #: Q5JU85; O60275; Q5JUX1; B3KT97; C7SDG1
• Molecular Weight: Predicted: 104; 133; 141; 164 kDa; ; Observed: 140 kDa
• NCBI Official Full Name: IQ motif and SEC7 domain-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: IQ motif and Sec7 domain 2
• NCBI Official Symbol: IQSEC2
• NCBI Official Synonym Symbols: MRX1; BRAG1
• NCBI Protein Information: IQ motif and SEC7 domain-containing protein 2; brefeldin A resistant Arf-guanine nucleotide exchange factor 1; brefeldin A resistant Arf-guanine nucleotide exchange factor 1b; brefeldin A resistant Arf-guanine nucleotide exchange factor 1c
• UniProt Protein Name: IQ motif and SEC7 domain-containing protein 2
• Protein Family: IQ motif and SEC7 domain-containing protein
• UniProt Gene Name: IQSEC2
• UniProt Synonym Gene Names: KIAA0522
• UniProt Entry Name: IQEC2_HUMAN

NCBI Description

This gene encodes a guanine nucleotide exchange factor for the ARF family of small GTP-binding proteins. The encoded protein is a component of the postsynaptic density at excitatory synapses, and may play a critical role in cytoskeletal and synaptic organization through the activation of selected ARF substrates including ARF1 and ARF6. Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

IQSEC2: Defects in IQSEC2 are the cause of mental retardation X- linked type 1 (MRX1). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation. Belongs to the BRAG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, ARF; GEFs

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: trans-Golgi network; cell junction; nucleus

Molecular Function: ARF guanyl-nucleotide exchange factor activity

Biological Process: vesicle-mediated transport; regulation of ARF protein signal transduction; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity

Disease: Mental Retardation, X-linked 1