HUS1B sirna

HUS1B siRNA (Human)

Cat. Num. AAA8233872

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HUS1B; HUS1B siRNA (Human); Checkpoint protein HUS1B; hHUS1B; HUS1B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HUS1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 278

• Applicable Applications for HUS1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HUS1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HUS1B sirna

siRNA to inhibit HUS1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31077215
• NCBI GeneID: 135458
• NCBI Accession #: NP_683762.2
• NCBI GenBank Nucleotide #: NM_148959.3
• UniProt Accession #: Q8NHY5; Q5T4Z2
• Molecular Weight: 30,982 Da
• NCBI Official Full Name: checkpoint protein HUS1B
• NCBI Official Synonym Full Names: HUS1 checkpoint clamp component B
• NCBI Official Symbol: HUS1B
• NCBI Protein Information: checkpoint protein HUS1B
• UniProt Protein Name: Checkpoint protein HUS1B
• Protein Family: Checkpoint protein
• UniProt Gene Name: HUS1B
• UniProt Synonym Gene Names: hHUS1B
• UniProt Entry Name: HUS1B_HUMAN

NCBI Description

The protein encoded by this gene is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the check point protein RAD1 but not with RAD9. Overexpression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to the HUS1. [provided by RefSeq, Jul 2008]

Uniprot Description

HUS1B: is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the check point protein RAD1 but not with RAD9. Overexpression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to the HUS1. [provided by RefSeq, Jul 2008]

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p25.3

Cellular Component: nucleolus

Biological Process: DNA damage checkpoint; DNA repair