HLA-DQA2 sirna

HLA-DQA2 siRNA (Human)

Cat. Num. AAA827946

• Gene Names: HLA-DQA2; HLA-DXA; DX-ALPHA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HLA-DQA2; HLA-DQA2 siRNA (Human); HLA-DXA; HLA class II histocompatibility antigen DQ alpha 2 chain; DX alpha chain; HLA class II histocompatibility antigen DQ(6) alpha chain; HLA-DQA1; MHC class II DQA2; HLA-DQA2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HLA-DQA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 255

• Applicable Applications for HLA-DQA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HLA-DQA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HLA-DQA2 sirna

siRNA to inhibit HLA-DQA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11095447
• NCBI GeneID: 3118
• NCBI Accession #: NP_064440.1
• NCBI GenBank Nucleotide #: NM_020056.4
• UniProt Accession #: P01906; O19789; Q5SQ94; Q5SR04; A2BF37; B0V0E7
• Molecular Weight: 28,033 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DQ alpha 2 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DQ alpha 2
• NCBI Official Symbol: HLA-DQA2
• NCBI Official Synonym Symbols: HLA-DXA; DX-ALPHA
• NCBI Protein Information: HLA class II histocompatibility antigen, DQ alpha 2 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DQ alpha 2 chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DQA2
• UniProt Synonym Gene Names: HLA-DXA
• UniProt Entry Name: DQA2_HUMAN

NCBI Description

This gene belongs to the HLA class II alpha chain family. The encoded protein forms a heterodimer with a class II beta chain. It is located in intracellular vesicles and plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells, macrophages) and are used to present antigenic peptides on the cell surface to be recognized by CD4 T-cells. [provided by RefSeq, Jun 2010]

Uniprot Description

HLA-DQA2: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; lysosomal membrane; integral to plasma membrane; plasma membrane; endosome membrane; trans-Golgi network membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; T cell receptor signaling pathway