Rabbit anti-Human BRMS1 Polyclonal Antibody | anti-BRMS1 antibody

BRMS1, NT (BRMS1, Breast cancer metastasis-suppressor 1) (FITC)

Cat. Num. AAA6278926

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BRMS1; Polyclonal Antibody; NT (BRMS1; Breast cancer metastasis-suppressor 1) (FITC); Breast cancer metastasis-suppressor 1; anti-BRMS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-BRMS1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BRMS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-77 amino acids from the N-terminal region of human BRMS1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BRMS1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BRMS1 antibody detected the BRMS1 protein (arrow).)

Related Product Information for anti-BRMS1 antibody

This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.

Product Categories/Family for anti-BRMS1 antibody

Antibodies; Cancer Markers

References

Wu, Y., et al. Cancer Lett. 293(1):82-91(2010). Vaidya, K.S., et al. Cancer Lett. 281(1):100-107(2009). Frolova, N., et al. Tumour Biol. 30(3):148-159(2009). Martins-de-Souza, D., et al. BMC Psychiatry 9, 17 (2009): Cicek, M., et al. Clin. Exp. Metastasis 26(3):229-237(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 17530785
• NCBI GeneID: 25855
• NCBI Accession #: NP_056214.1
• NCBI GenBank Nucleotide #: NM_015399.3
• UniProt Accession #: Q9HCU9; Q6IAI2
• Molecular Weight: 28,461 Da
• NCBI Official Full Name: breast cancer metastasis-suppressor 1 isoform 1
• NCBI Official Synonym Full Names: breast cancer metastasis suppressor 1
• NCBI Official Symbol: BRMS1
• NCBI Protein Information: breast cancer metastasis-suppressor 1
• UniProt Protein Name: Breast cancer metastasis-suppressor 1
• Protein Family: Breast cancer metastasis-suppressor
• UniProt Gene Name: BRMS1
• UniProt Entry Name: BRMS1_HUMAN

NCBI Description

This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

BRMS1: Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down- regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma. Belongs to the BRMS1 family.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: NF-kappaB binding; protein binding; histone deacetylase binding; histone deacetylase activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; inhibition of NF-kappaB transcription factor; apoptosis; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent