Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of extracts from K562 cells, using DGKE antibody.)

Rabbit DGKE Polyclonal Antibody | anti-DGKE antibody

DGKE Antibody

Gene Names
DGKE; DGK; DAGK5; DAGK6; NPHS7
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DGKE; Polyclonal Antibody; DGKE Antibody; Diacylglycerol kinase epsilon; EC 2.7.1.107; Diglyceride kinase epsilon; DGK-epsilon; DAG kinase epsilon; anti-DGKE antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total DGKE protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
567
Applicable Applications for anti-DGKE antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human DGKE.
Target Name
DGKE
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from K562 cells, using DGKE antibody.)

Western Blot (WB) (Western blot analysis of extracts from K562 cells, using DGKE antibody.)
Related Product Information for anti-DGKE antibody
Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.Tang W., J. Biol. Chem. 271:10237-10241(1996).Tang W., Gene 239:185-192(1999).Sjoeblom T., Science 314:268-274(2006).
Product Categories/Family for anti-DGKE antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,452 Da
NCBI Official Full Name
diacylglycerol kinase epsilon
NCBI Official Synonym Full Names
diacylglycerol kinase, epsilon 64kDa
NCBI Official Symbol
DGKE
NCBI Official Synonym Symbols
DGK; DAGK5; DAGK6; NPHS7
NCBI Protein Information
diacylglycerol kinase epsilon
UniProt Protein Name
Diacylglycerol kinase epsilon
Protein Family
UniProt Gene Name
DGKE
UniProt Synonym Gene Names
DAGK5; DAG kinase epsilon; DGK-epsilon
UniProt Entry Name
DGKE_HUMAN

NCBI Description

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKE: Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Kinase, lipid; Lipid Metabolism - glycerophospholipid; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; EC 2.7.1.107

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: membrane; cytoplasm; plasma membrane; integral to membrane

Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation; phospholipid biosynthetic process

Disease: Nephrotic Syndrome, Type 7

Research Articles on DGKE

Similar Products

Product Notes

The DGKE dgke (Catalog #AAA9409496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGKE Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DGKE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the DGKE dgke for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.