HDAC9 sirna

HDAC9 siRNA (Human)

Cat. Num. AAA827625

• Gene Names: HDAC9; HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HDAC9; HDAC9 siRNA (Human); HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR; HDAC9 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HDAC9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 588

• Applicable Applications for HDAC9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommend transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HDAC9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Store at -20°C for one year.

Related Product Information for HDAC9 sirna

siRNA to inhibit HDAC9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 323423044
• NCBI GeneID: 9734
• NCBI Accession #: NP_001191073.1
• NCBI GenBank Nucleotide #: NM_001204144.1
• UniProt Accession #: Q9UKV0; O94845; O95028; Q2M2R6; A7E2F3; B7Z4I4; B7Z917; B7Z928; B7Z940; C9JS87; E7EX34; F8W9E0
• NCBI Official Full Name: histone deacetylase 9 isoform 6
• NCBI Official Synonym Full Names: histone deacetylase 9
• NCBI Official Symbol: HDAC9
• NCBI Official Synonym Symbols: HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL
• NCBI Protein Information: histone deacetylase 9
• UniProt Protein Name: Histone deacetylase 9
• UniProt Gene Name: HDAC9
• UniProt Synonym Gene Names: HDAC7; HDAC7B; HDRP; KIAA0744; MITR; HD9; HD7; HD7b
• UniProt Entry Name: HDAC9_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC9: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Protein type: Deacetylase; Transcription, coactivator/corepressor; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: nucleoplasm; transcription factor complex; histone methyltransferase complex; cytoplasm; histone deacetylase complex; nucleus

Molecular Function: protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; transcription factor binding

Biological Process: regulation of skeletal muscle fiber development; Notch signaling pathway; transcription, DNA-dependent; B cell activation; cellular response to insulin stimulus; regulation of striated muscle cell differentiation; B cell differentiation; heart development; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inflammatory response