GOT2 sirna

GOT2 siRNA (Human)

Cat. Num. AAA8210200

• Gene Names: GOT2; KAT4; KATIV; mitAAT
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GOT2; GOT2 siRNA (Human); Aspartate aminotransferase mitochondrial; mAspAT; Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A; GOT2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GOT2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 387

• Applicable Applications for GOT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GOT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GOT2 sirna

siRNA to inhibit GOT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 555290167
• NCBI GeneID: 2806
• NCBI Accession #: NP_001273149.1
• NCBI GenBank Nucleotide #: NM_001286220.1
• UniProt Accession #: P00505; Q53FL3; Q9BWA3; B4DJA6; E7ERW2
• Molecular Weight: 43,030 Da
• NCBI Official Full Name: aspartate aminotransferase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 2, mitochondrial
• NCBI Official Symbol: GOT2
• NCBI Official Synonym Symbols: KAT4; KATIV; mitAAT
• NCBI Protein Information: aspartate aminotransferase, mitochondrial
• UniProt Protein Name: Aspartate aminotransferase, mitochondrial
• UniProt Gene Name: GOT2
• UniProt Synonym Gene Names: mAspAT; FABP-1; FABPpm
• UniProt Entry Name: AATM_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - arginine and proline; Mitochondrial; EC 2.6.1.1; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.7; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - cysteine and methionine; Transferase

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane

Molecular Function: kynurenine-oxoglutarate transaminase activity; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; aspartate biosynthetic process; glucose metabolic process; pathogenesis; 2-oxoglutarate metabolic process; gluconeogenesis; response to ethanol; 4-hydroxyproline catabolic process; carbohydrate metabolic process; aspartate metabolic process; aspartate catabolic process; glutamate catabolic process to 2-oxoglutarate; amino acid biosynthetic process; fatty acid transport