Western Blot (WB)
(Western blot analysis of GOT2 expression in HepG2 (A), Jurkat (B), mouse liver (C), mouse brain (D), rat kidney (E) whole cell lysates.)
GOT2 Polyclonal Antibody | anti-GOT2 antibody
Anti-GOT2 Antibody
Western Blot (WB)
(Western blot analysis of GOT2 expression in HepG2 (A), Jurkat (B), mouse liver (C), mouse brain (D), rat kidney (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GOT2 expression in HepG2 (A), Jurkat (B), mouse liver (C), mouse brain (D), rat kidney (E) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GOT2 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GOT2 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
Uniprot Description
GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Mitochondrial; EC 2.6.1.7; EC 2.6.1.1; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - arginine and proline; Transferase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine
Chromosomal Location of Human Ortholog: 16q21
Cellular Component: cell surface; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; myelin sheath; perikaryon; plasma membrane; protein complex
Molecular Function: amino acid binding; aspartate transaminase activity; enzyme binding; identical protein binding; kynurenine-oxoglutarate transaminase activity; phospholipid binding; protein homodimerization activity; pyridoxal phosphate binding
Biological Process: 2-oxoglutarate metabolic process; 4-hydroxyproline catabolic process; amino acid biosynthetic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; fatty acid transport; gluconeogenesis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glutamate metabolic process; oxaloacetate metabolic process; response to ethanol
Research Articles on GOT2
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Product Notes
The GOT2 got2 (Catalog #AAA8245284) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-GOT2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GOT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200. Researchers should empirically determine the suitability of the GOT2 got2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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