GNB1L sirna

GNB1L siRNA (Human)

Cat. Num. AAA8240138

• Gene Names: GNB1L; GY2; FKSG1; WDR14; WDVCF; DGCRK3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GNB1L; GNB1L siRNA (Human); GY2; KIAA1645; WDR14; Guanine nucleotide-binding protein subunit beta-like protein 1; G protein subunit beta-like protein 1; DGCRK3; WD repeat-containing protein 14; WD40 repeat-containing protein deleted in VCFS; WDVCF; GNB1L sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GNB1L siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 327

• Applicable Applications for GNB1L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GNB1L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GNB1L sirna

siRNA to inhibit GNB1L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17402902
• NCBI GeneID: 54584
• NCBI Accession #: NP_443730.1
• NCBI GenBank Nucleotide #: NM_053004.2
• UniProt Accession #: Q9BYB4; Q9H2S2; Q9H4M4
• Molecular Weight: 22,896 Da
• NCBI Official Full Name: guanine nucleotide-binding protein subunit beta-like protein 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 1-like
• NCBI Official Symbol: GNB1L
• NCBI Official Synonym Symbols: GY2; FKSG1; WDR14; WDVCF; DGCRK3
• NCBI Protein Information: guanine nucleotide-binding protein subunit beta-like protein 1
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-like protein 1
• Protein Family: Guanine nucleotide-binding protein subunit beta-like protein
• UniProt Gene Name: GNB1L
• UniProt Synonym Gene Names: GY2; KIAA1645; WDR14; G protein subunit beta-like protein 1; WDVCF
• UniProt Entry Name: GNB1L_HUMAN

NCBI Description

This gene encodes a G-protein beta-subunit-like polypeptide which is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 6 WD repeats and is highly expressed in the heart. The gene maps to the region on chromosome 22q11, which is deleted in DiGeorge syndrome, trisomic in derivative 22 syndrome and tetrasomic in cat-eye syndrome. Therefore, this gene may contribute to the etiology of those disorders. Transcripts from this gene share exons with some transcripts from the C22orf29 gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GNB1L: 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: internal side of plasma membrane; cytoplasm

Biological Process: G-protein coupled receptor protein signaling pathway; social behavior