Human Anterior Gradient Protein 2 (AGR2) ELISA Kit | AGR2 elisa kit

Human Anterior Gradient Protein 2 (AGR2) ELISA Kit

Cat. Num. AAA2704331

• Gene Names: AGR2; AG2; AG-2; HPC8; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
• Reactivity: Human
• Synonyms: Anterior Gradient Protein 2 (AGR2); Human Anterior Gradient Protein 2 (AGR2) ELISA Kit; GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Protein Disulfide Isomerase Family A; Member 17; Secreted cement gland protein XAG-2 homolog; AGR2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anterior Gradient Protein 2 (AGR2).; No significant cross-reactivity or interference between Anterior Gradient Protein 2 (AGR2) and analogues was observed.
• Sequence Length: 175

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.4pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anterior Gradient Protein 2 (AGR2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anterior Gradient Protein 2 (AGR2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGR2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient Protein 2 (AGR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anterior Gradient Protein 2 (AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anterior Gradient Protein 2 (AGR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anterior Gradient Protein 2 (AGR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5453541
• NCBI GeneID: 10551
• NCBI Accession #: NP_006399.1
• NCBI GenBank Nucleotide #: NM_006408.3
• UniProt Accession #: O95994
• Molecular Weight: 19,979 Da
• NCBI Official Full Name: anterior gradient protein 2 homolog
• NCBI Official Synonym Full Names: anterior gradient 2, protein disulphide isomerase family member
• NCBI Official Symbol: AGR2
• NCBI Official Synonym Symbols: AG2; AG-2; HPC8; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
• NCBI Protein Information: anterior gradient protein 2 homolog
• UniProt Protein Name: Anterior gradient protein 2 homolog
• Protein Family: Anterior gradient protein
• UniProt Gene Name: AGR2
• UniProt Synonym Gene Names: AG2; AG-2; hAG-2

NCBI Description

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and a C-terminal ER-retention sequence. This protein plays a role in cell migration, cellular transformation and metastasis and is as a p53 inhibitor. As an ER-localized molecular chaperone, it plays a role in the folding, trafficking, and assembly of cysteine-rich transmembrane receptors and the cysteine-rich intestinal gylcoprotein mucin. This gene has been implicated in inflammatory bowel disease and cancer progression. [provided by RefSeq, Mar 2017]

Uniprot Description

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed:23274113).