GDF10 sirna

GDF10 siRNA (Human)

Cat. Num. AAA8236443

• Gene Names: GDF10; BMP3B; BMP-3b
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GDF10; GDF10 siRNA (Human); BMP3B; Bone morphogenetic protein 3B; BMP-3B; Bone-inducing protein; BIP; Growth/differentiation factor 10; GDF-10; GDF10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GDF10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 478

• Applicable Applications for GDF10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GDF10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GDF10 sirna

siRNA to inhibit GDF10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4826740
• NCBI GeneID: 2662
• NCBI Accession #: NP_004953.1
• NCBI GenBank Nucleotide #: NM_004962.3
• UniProt Accession #: P55107; Q5VSQ8; Q9UCX6
• Molecular Weight: 53,122 Da
• NCBI Official Full Name: growth/differentiation factor 10
• NCBI Official Synonym Full Names: growth differentiation factor 10
• NCBI Official Symbol: GDF10
• NCBI Official Synonym Symbols: BMP3B; BMP-3b
• NCBI Protein Information: growth/differentiation factor 10
• UniProt Protein Name: Growth/differentiation factor 10
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF10
• UniProt Synonym Gene Names: GDF-10; BMP-3B; BIP
• UniProt Entry Name: GDF10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF10: is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: fat cell differentiation; regulation of apoptosis; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; cell development; skeletal development; growth