Human Neural Cell Adhesion Molecule ELISA Kit | CD56 elisa kit

Human Neural Cell Adhesion Molecule ELISA Kit

Cat. Num. AAA764443

• Gene Names: NCAM1; CD56; NCAM; MSK39
• Reactivity: Human
• Synonyms: Neural Cell Adhesion Molecule; Human Neural Cell Adhesion Molecule ELISA Kit; CD56/NCAM-1/CD56 antigen/MSK39/N-CAM-1/NCAMantigen recognized by monoclonal 5.1H11/neural cell adhesion molecule 1/neural cell adhesion molecule; NCAM; CD56 elisa kit

• Reactivity: Human
• Sequence Length: 23

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CD56 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CD56/NCAM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CD56/NCAM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD56/NCAM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CD56/NCAM can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 241117
• NCBI GeneID: 4684
• Molecular Weight: 40,760 Da
• NCBI Official Full Name: neural cell adhesion molecule, partial
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: NCAM1
• NCBI Official Synonym Symbols: CD56; NCAM; MSK39
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: NCAM1
• UniProt Synonym Gene Names: NCAM; N-CAM-1; NCAM-1
• UniProt Entry Name: NCAM1_HUMAN

NCBI Description

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; Golgi membrane; integral to membrane; membrane; plasma membrane

Molecular Function: identical protein binding; viral receptor activity

Biological Process: activation of MAPKK activity; axon guidance; cell adhesion; cytokine and chemokine mediated signaling pathway; entry of virus into host cell; epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway