G6PC3 sirna

G6PC3 siRNA (Human)

Cat. Num. AAA8230143

• Gene Names: G6PC3; SCN4; UGRP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: G6PC3; G6PC3 siRNA (Human); UGRP; Glucose-6-phosphatase 3; G-6-Pase 3; G6Pase 3; Glucose-6-phosphatase beta; G6Pase-beta; Ubiquitous glucose-6-phosphatase catalytic subunit-related protein; G6PC3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: G6PC3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 346

• Applicable Applications for G6PC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human G6PC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for G6PC3 sirna

siRNA to inhibit G6PC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 24308434
• NCBI GeneID: 92579
• NCBI Accession #: NP_612396.1
• NCBI GenBank Nucleotide #: NM_138387.3
• UniProt Accession #: Q9BUM1; Q8WU15
• Molecular Weight: 38,735 Da
• NCBI Official Full Name: glucose-6-phosphatase 3
• NCBI Official Synonym Full Names: glucose 6 phosphatase, catalytic, 3
• NCBI Official Symbol: G6PC3
• NCBI Official Synonym Symbols: SCN4; UGRP
• NCBI Protein Information: glucose-6-phosphatase 3
• UniProt Protein Name: Glucose-6-phosphatase 3
• Protein Family: Glucose-6-phosphatase
• UniProt Gene Name: G6PC3
• UniProt Synonym Gene Names: UGRP; G-6-Pase 3; G6Pase 3; G6Pase-beta
• UniProt Entry Name: G6PC3_HUMAN

NCBI Description

This gene encodes the catalytic subunit of glucose-6-phosphatase (G6Pase). G6Pase is located in the endoplasmic reticulum (ER) and catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the last step of the gluconeogenic and glycogenolytic pathways. Mutations in this gene result in autosomal recessive severe congenital neutropenia. Multiple transcript variants have been found for this gene, only one of which is expected to express a protein.[provided by RefSeq, Sep 2009]

Uniprot Description

G6PC3: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function. Defects in G6PC3 are the cause of neutropenia severe congenital autosomal recessive type 4 (SCN4). Autosomal recessive SCN constitutes a primary immunodeficiency syndrome associated with increased apoptosis in myeloid cells. Individuals show a paucity of mature neutrophils in peripheral blood and bone marrow and develop life-threatening bacterial infections. SCN4 is a severe congenital neutropenia syndrome associated with cardiac and urogenital malformations. Defects in G6PC3 are the cause of Dursun syndrome (DURSS). A disease characterized by pulmonary arterial hypertension, cardiac abnormalities including secundum-type atrial septal defect, intermittent neutropenia, lymphopenia, monocytosis and anemia. Belongs to the glucose-6-phosphatase family.

Protein type: Phosphatase (non-protein); Transporter, SLC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass; EC 3.1.3.9

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: glucose-6-phosphatase activity

Biological Process: dephosphorylation; glucose-6-phosphate transport; hexose transport; carbohydrate metabolic process; glucose 6-phosphate metabolic process; pathogenesis; glucose transport; transmembrane transport; gluconeogenesis

Disease: Neutropenia, Severe Congenital, 4, Autosomal Recessive