FNTB sirna

FNTB siRNA (Human)

Cat. Num. AAA8239120

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FNTB; FNTB siRNA (Human); Protein farnesyltransferase subunit beta; FTase-beta; CAAX farnesyltransferase subunit beta; Ras proteins prenyltransferase subunit beta; FNTB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FNTB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 391

• Applicable Applications for FNTB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FNTB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FNTB sirna

siRNA to inhibit FNTB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 321400144
• NCBI GeneID: 100529261
• NCBI Accession #: NP_001189487.1
• NCBI GenBank Nucleotide #: NM_001202558.1
• UniProt Accession #: P49356; B2RDX6; B4E1A0
• Molecular Weight: 43,574 Da
• NCBI Official Full Name: CHURC1-FNTB protein isoform 2
• NCBI Official Synonym Full Names: CHURC1-FNTB readthrough
• NCBI Official Symbol: CHURC1-FNTB
• NCBI Protein Information: CHURC1-FNTB protein
• UniProt Protein Name: Protein farnesyltransferase subunit beta
• UniProt Gene Name: FNTB
• UniProt Synonym Gene Names: FTase-beta
• UniProt Entry Name: FNTB_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring CHURC1 (churchill domain containing 1) and FNTB (farnesyltransferase, CAAX box, beta) on chromosome 14. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]

Uniprot Description

FNTB: a subunit of farnesyltransferase that is responsible for peptide-binding. Farnesyltransferase catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins.

Protein type: Transferase; EC 2.5.1.58

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: microtubule associated complex; cytosol

Molecular Function: protein binding; farnesyltranstransferase activity; isoprenoid binding; zinc ion binding; drug binding; peptide binding; protein farnesyltransferase activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; protein farnesylation; response to inorganic substance; regulation of rhodopsin mediated signaling; wound healing; response to cytokine stimulus; positive regulation of cell cycle; response to organic cyclic substance; positive regulation of nitric-oxide synthase biosynthetic process