ENPP1 sirna

ENPP1 siRNA (Human)

Cat. Num. AAA8205152

• Gene Names: ENPP1; M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ENPP1; ENPP1 siRNA (Human); M6S1; NPPS; PC1; PDNP1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Membrane component chromosome 6 surface marker 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1; ENPP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ENPP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 925

• Applicable Applications for ENPP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ENPP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ENPP1 sirna

siRNA to inhibit ENPP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 170650661
• NCBI GeneID: 5167
• NCBI Accession #: NP_006199.2
• NCBI GenBank Nucleotide #: NM_006208.2
• UniProt Accession #: P22413; Q5T9R6; Q9NPZ3; Q9P1P6; Q9UP61; Q9Y6K3
• Molecular Weight: 104,924 Da
• NCBI Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 1
• NCBI Official Symbol: ENPP1
• NCBI Official Synonym Symbols: M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Protein Family: Ectonucleotide pyrophosphatase/phosphodiesterase family
• UniProt Gene Name: ENPP1
• UniProt Synonym Gene Names: M6S1; NPPS; PC1; PDNP1; E-NPP 1; NPPase
• UniProt Entry Name: ENPP1_HUMAN

NCBI Description

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Membrane protein, integral; Cofactor and Vitamin Metabolism - riboflavin; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Carbohydrate Metabolism - starch and sucrose; Phosphatase (non-protein); Nucleotide Metabolism - purine; Motility/polarity/chemotaxis; EC 3.1.4.1; Phosphodiesterase; EC 3.6.1.9

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: extracellular space; cell surface; lysosomal membrane; basolateral plasma membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; protein homodimerization activity; 3'-phosphoadenosine 5'-phosphosulfate binding; zinc ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; insulin receptor binding; polysaccharide binding; protein binding; nucleic acid binding; scavenger receptor activity; ATP binding

Biological Process: receptor-mediated endocytosis; sequestering of triacylglycerol; generation of precursor metabolites and energy; vitamin metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; bone remodeling; negative regulation of fat cell differentiation; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; riboflavin metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; negative regulation of protein amino acid autophosphorylation; immune response; negative regulation of cell growth; regulation of bone mineralization; water-soluble vitamin metabolic process; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process

Disease: Obesity; Cole Disease; Arterial Calcification, Generalized, Of Infancy, 1; Hypophosphatemic Rickets, Autosomal Recessive, 2; Diabetes Mellitus, Noninsulin-dependent