EHMT1 sirna

EHMT1 siRNA (Human)

Cat. Num. AAA8200056

• Gene Names: EHMT1; GLP; GLP1; KMT1D; FP13812; EUHMTASE1; Eu-HMTase1; bA188C12.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EHMT1; EHMT1 siRNA (Human); EUHMTASE1; GLP; KIAA1876; KMT1D; Histone-lysine N-methyltransferase EHMT1; Euchromatic histone-lysine N-methyltransferase 1; Eu-HMTase1; G9a-like protein 1; GLP1; Histone H3-K9 methyltransferase 5; H3-K9-HMTase 5; Lysine N-methyltransferase 1D; EHMT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EHMT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 808

• Applicable Applications for EHMT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EHMT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EHMT1 sirna

siRNA to inhibit EHMT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224465235
• NCBI GeneID: 79813
• NCBI Accession #: NP_001138999.1
• NCBI GenBank Nucleotide #: NM_001145527.1
• UniProt Accession #: Q9H9B1; Q86X08; Q8TCN7; Q96F53; Q96JF1; Q96KH4; B1AQ58; B1AQ59
• Molecular Weight: 86,704 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase EHMT1 isoform 2
• NCBI Official Synonym Full Names: euchromatic histone-lysine N-methyltransferase 1
• NCBI Official Symbol: EHMT1
• NCBI Official Synonym Symbols: GLP; GLP1; KMT1D; FP13812; EUHMTASE1; Eu-HMTase1; bA188C12.1
• NCBI Protein Information: histone-lysine N-methyltransferase EHMT1
• UniProt Protein Name: Histone-lysine N-methyltransferase EHMT1
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: EHMT1
• UniProt Synonym Gene Names: EUHMTASE1; GLP; KIAA1876; KMT1D; Eu-HMTase1; GLP; GLP1; H3-K9-HMTase 5
• UniProt Entry Name: EHMT1_HUMAN

NCBI Description

The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

EHMT1: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome). Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems. Belongs to the histone-lysine methyltransferase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; chromosome; nucleus

Molecular Function: methyltransferase activity; protein binding; zinc ion binding; p53 binding; histone lysine N-methyltransferase activity (H3-K9 specific); protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: histone methylation; embryonic development; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; DNA methylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; histone H3-K9 methylation

Disease: Kleefstra Syndrome