DYRK1B sirna

DYRK1B siRNA (Human)

Cat. Num. AAA8239523

• Gene Names: DYRK1B; MIRK; AOMS3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DYRK1B; DYRK1B siRNA (Human); MIRK; Dual specificity tyrosine-phosphorylation-regulated kinase 1B; Minibrain-related kinase; Mirk protein kinase; DYRK1B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DYRK1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 629

• Applicable Applications for DYRK1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DYRK1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DYRK1B sirna

siRNA to inhibit DYRK1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4758222
• NCBI GeneID: 9149
• NCBI Accession #: NP_004705.1
• NCBI GenBank Nucleotide #: NM_004714.2
• UniProt Accession #: Q9Y463; O75258; O75788; O75789
• Molecular Weight: 66,336 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 1B isoform p69
• NCBI Official Synonym Full Names: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B
• NCBI Official Symbol: DYRK1B
• NCBI Official Synonym Symbols: MIRK; AOMS3
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1B
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1B
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: DYRK1B
• UniProt Synonym Gene Names: MIRK
• UniProt Entry Name: DYR1B_HUMAN

NCBI Description

This gene encodes a member of a family of nuclear-localized protein kinases. The encoded protein participates in the regulation of the cell cycle. Expression of this gene may be altered in tumor cells, and mutations in this gene were found to cause abdominal obesity-metabolic syndrome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

DYRK1B: a dual-specificity protein kinase of the DYRK family. Localizes in the nucleus, where it enhances the transcriptional activity of TCF1/HNF1A. A co-activator of FKHR (FOXO1a)-dependent glucose-6-phosphatase gene expression. Induced by members of the Rho-family in myoblasts. Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines. Contains a bipartite nuclear targeting signal sequence. Three splice-variant isoforms have been described.

Protein type: Kinase, protein; EC 2.7.12.1; Protein kinase, CMGC; Protein kinase, dual-specificity (non-receptor); CMGC group; DYRK family; Dyrk1 subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transcription coactivator activity; protein serine/threonine/tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; myoblast fusion

Disease: Abdominal Obesity-metabolic Syndrome 3