DPM2 sirna

DPM2 siRNA (Human)

Cat. Num. AAA8236879

• Gene Names: DPM2; CDG1U
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DPM2; DPM2 siRNA (Human); Dolichol phosphate-mannose biosynthesis regulatory protein; Dolichol-phosphate mannose synthase subunit 2; DPM synthase subunit 2; DPM2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DPM2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 84

• Applicable Applications for DPM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DPM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DPM2 sirna

siRNA to inhibit DPM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503365
• NCBI GeneID: 8818
• NCBI Accession #: NP_003854.1
• NCBI GenBank Nucleotide #: NM_003863.3
• UniProt Accession #: O94777; Q5XKK9; Q6FGH3
• Molecular Weight: 9,312 Da
• NCBI Official Full Name: dolichol phosphate-mannose biosynthesis regulatory protein
• NCBI Official Synonym Full Names: dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit
• NCBI Official Symbol: DPM2
• NCBI Official Synonym Symbols: CDG1U
• NCBI Protein Information: dolichol phosphate-mannose biosynthesis regulatory protein
• UniProt Protein Name: Dolichol phosphate-mannose biosynthesis regulatory protein
• Protein Family: Dolichol phosphate-mannose biosynthesis regulatory protein
• UniProt Gene Name: DPM2
• UniProt Synonym Gene Names: DPM synthase subunit 2
• UniProt Entry Name: DPM2_HUMAN

NCBI Description

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1. [provided by RefSeq, Jul 2008]

Uniprot Description

DPM2: Regulates the biosynthesis of dolichol phosphate- mannose. Essential for the ER localization and stable expression of DPM1. Belongs to the DPM2 family.

Protein type: Membrane protein, integral; Transferase; Membrane protein, multi-pass; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: endoplasmic reticulum membrane; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; perinuclear region of cytoplasm; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; dolichyl-phosphate beta-D-mannosyltransferase activity; enzyme regulator activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid O-linked mannosylation; regulation of catalytic activity; preassembly of GPI anchor in ER membrane; GPI anchor biosynthetic process; regulation of protein stability; protein amino acid N-linked glycosylation via asparagine; dolichol metabolic process; C-terminal protein lipidation; post-translational protein modification

Disease: Congenital Disorder Of Glycosylation, Type Iu