DLGAP2 sirna

DLGAP2 siRNA (Human)

Cat. Num. AAA8232731

• Gene Names: DLGAP2; DAP2; SAPAP2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLGAP2; DLGAP2 siRNA (Human); DAP2; Disks large-associated protein 2; DAP-2; PSD-95/SAP90-binding protein 2; SAP90/PSD-95-associated protein 2; SAPAP2; DLGAP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DLGAP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 961

• Applicable Applications for DLGAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DLGAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLGAP2 sirna

siRNA to inhibit DLGAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 461496466
• NCBI GeneID: 9228
• NCBI Accession #: NP_001264090.1
• NCBI GenBank Nucleotide #: NM_001277161.1
• UniProt Accession #: Q9P1A6; O14488; O14664; Q9P1A7; A1QCF8; A1QCF9; A5D8Y2
• Molecular Weight: 108,908 Da
• NCBI Official Full Name: disks large-associated protein 2 isoform 2
• NCBI Official Synonym Full Names: discs, large (Drosophila) homolog-associated protein 2
• NCBI Official Symbol: DLGAP2
• NCBI Official Synonym Symbols: DAP2; SAPAP2
• NCBI Protein Information: disks large-associated protein 2
• UniProt Protein Name: Disks large-associated protein 2
• Protein Family: Disks large-associated protein
• UniProt Gene Name: DLGAP2
• UniProt Synonym Gene Names: DAP2; DAP-2; SAPAP2
• UniProt Entry Name: DLGP2_HUMAN

NCBI Description

The product of this gene is a membrane-associated protein that may play a role in synapse organization and signalling in neuronal cells. This gene is biallelically expressed in the brain, however, only the paternal allele is expressed in the testis (PMID:18055845). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2014]

Uniprot Description

SAPAP2: May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane. Belongs to the SAPAP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other

Chromosomal Location of Human Ortholog: 8p23

Cellular Component: postsynaptic membrane; postsynaptic density; neurofilament; cell junction

Molecular Function: protein binding

Biological Process: nerve-nerve synaptic transmission