DIRAS3 sirna

DIRAS3 siRNA (Human)

Cat. Num. AAA8216066

• Gene Names: DIRAS3; ARHI; NOEY2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DIRAS3; DIRAS3 siRNA (Human); ARHI; NOEY2; RHOI; GTP-binding protein Di-Ras3; Distinct subgroup of the Ras family member 3; Rho-related GTP-binding protein RhoI; DIRAS3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DIRAS3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 229

• Applicable Applications for DIRAS3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DIRAS3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DIRAS3 sirna

siRNA to inhibit DIRAS3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4757772
• NCBI GeneID: 9077
• NCBI Accession #: NP_004666.1
• NCBI GenBank Nucleotide #: NM_004675.2
• UniProt Accession #: O95661; B3KMP3
• Molecular Weight: 25,861 Da
• NCBI Official Full Name: GTP-binding protein Di-Ras3
• NCBI Official Synonym Full Names: DIRAS family, GTP-binding RAS-like 3
• NCBI Official Symbol: DIRAS3
• NCBI Official Synonym Symbols: ARHI; NOEY2
• NCBI Protein Information: GTP-binding protein Di-Ras3
• UniProt Protein Name: GTP-binding protein Di-Ras3
• Protein Family: GTP-binding protein
• UniProt Gene Name: DIRAS3
• UniProt Synonym Gene Names: ARHI; NOEY2; RHOI
• UniProt Entry Name: DIRA3_HUMAN

NCBI Description

This gene is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]

Uniprot Description

DIRAS3: a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]

Protein type: G protein; G protein, monomeric; G protein, monomeric, di-Ras

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: plasma membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: genetic imprinting; small GTPase mediated signal transduction; regulation of cyclin-dependent protein kinase activity