DHTKD1 sirna

DHTKD1 siRNA (Human)

Cat. Num. AAA8233438

• Gene Names: DHTKD1; CMT2Q; AMOXAD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DHTKD1; DHTKD1 siRNA (Human); KIAA1630; Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1 mitochondrial; Dehydrogenase E1 and transketolase domain-containing protein 1; DHTKD1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DHTKD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 919

• Applicable Applications for DHTKD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DHTKD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DHTKD1 sirna

siRNA to inhibit DHTKD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38788380
• NCBI GeneID: 55526
• NCBI Accession #: NP_061176.3
• NCBI GenBank Nucleotide #: NM_018706.6
• UniProt Accession #: Q96HY7; Q68CU5; Q9BUM8; Q9HCE2
• Molecular Weight: 103,077 Da
• NCBI Official Full Name: probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
• NCBI Official Synonym Full Names: dehydrogenase E1 and transketolase domain containing 1
• NCBI Official Symbol: DHTKD1
• NCBI Official Synonym Symbols: CMT2Q; AMOXAD
• NCBI Protein Information: probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
• UniProt Protein Name: Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
• Protein Family: Probable 2-oxoglutarate dehydrogenase E1 component
• UniProt Gene Name: DHTKD1
• UniProt Synonym Gene Names: KIAA1630
• UniProt Entry Name: DHTK1_HUMAN

NCBI Description

This gene encodes a component of a mitochondrial 2-oxoglutarate-dehydrogenase-complex-like protein involved in the degradation pathways of several amino acids, including lysine. Mutations in this gene are associated with 2-aminoadipic 2-oxoadipic aciduria and Charcot-Marie-Tooth Disease Type 2Q. [provided by RefSeq, May 2013]

Uniprot Description

DHTKD1: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Belongs to the alpha-ketoglutarate dehydrogenase family.

Protein type: Oxidoreductase; EC 1.2.4.2

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: mitochondrion; oxoglutarate dehydrogenase complex; cytosol

Molecular Function: thiamin pyrophosphate binding; oxoglutarate dehydrogenase (succinyl-transferring) activity

Biological Process: generation of precursor metabolites and energy; glycolysis; tricarboxylic acid cycle; hemopoietic progenitor cell differentiation

Disease: Charcot-marie-tooth Disease, Axonal, Type 2q; 2-aminoadipic 2-oxoadipic Aciduria