DERL1 sirna

DERL1 siRNA (Human)

Cat. Num. AAA8231144

• Gene Names: DERL1; DER1; DER-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DERL1; DERL1 siRNA (Human); DER1; Derlin-1; Degradation in endoplasmic reticulum protein 1; DERtrin-1; Der1-like protein 1; DERL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DERL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 231

• Applicable Applications for DERL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DERL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DERL1 sirna

siRNA to inhibit DERL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 197927278
• NCBI GeneID: 79139
• NCBI Accession #: NP_001128143.1
• NCBI GenBank Nucleotide #: NM_001134671.2
• UniProt Accession #: Q9BUN8; B3KW41; E9PH19
• Molecular Weight: 26,423 Da
• NCBI Official Full Name: derlin-1 isoform b
• NCBI Official Synonym Full Names: derlin 1
• NCBI Official Symbol: DERL1
• NCBI Official Synonym Symbols: DER1; DER-1
• NCBI Protein Information: derlin-1
• UniProt Protein Name: Derlin-1
• Protein Family: Probable derlin
• UniProt Gene Name: DERL1
• UniProt Synonym Gene Names: DER1; DERtrin-1
• UniProt Entry Name: DERL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the derlin family. Members of this family participate in the ER-associated degradation response and retrotranslocate misfolded or unfolded proteins from the ER lumen to the cytosol for proteasomal degradation. This protein recognizes substrate in the ER and works in a complex to retrotranslocate it across the ER membrane into the cytosol. This protein may select cystic fibrosis transmembrane conductance regulator protein (CFTR) for degradation as well as unfolded proteins in Alzheimer's disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

DERL1: Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the degradation substrate. In case of infection by cytomegaloviruses, it plays a central role in the export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Also participates in the degradation process of misfolded cytomegalovirus US2 protein. Forms homo- and heterooligomers with DERL2 and DERL3; binding to DERL3 is poorer than that between DERL2 and DERL3. Interacts with AMFR, VIMP/SELS, SEL1L, SYVN1 and VCP, as well as with SEL1L-SYVN1 and VCP-VIMP protein complexes; this interaction is weaker than that observed between DERL2 and these complexes. Interacts with the cytomegalovirus US11 protein. Interacts with NGLY1 and YOD1. Does not bind to EDEM1. Interacts with RNF103. Up-regulated in response to endoplasmic reticulum stress via the ERN1-XBP1 pathway of the unfolded protein response (UPR). Ubiquitous. Belongs to the derlin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral; Chaperone

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: membrane; endoplasmic reticulum; early endosome; late endosome; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; protease binding; MHC class I protein binding; receptor activity

Biological Process: retrograde protein transport, ER to cytosol; intracellular transport of viral proteins in host cell; establishment of protein localization; ER-associated protein catabolic process; protein destabilization; unfolded protein response; response to unfolded protein