Rabbit DERL1 Polyclonal Antibody | anti-DERL1 antibody

DERL1 Antibody (C-term)

Cat. Num. AAA9208166

• Gene Names: DERL1; DER1; DER-1
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DERL1; Polyclonal Antibody; DERL1 Antibody (C-term); Derlin-1; Degradation in endoplasmic reticulum protein 1; DERtrin-1; Der1-like protein 1; DER1; anti-DERL1 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DERL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human DERL1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 224-251
• Sequence Length: 231

• Applicable Applications for anti-DERL1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of DERL1 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). DERL1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DERL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DERL1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DERL1 antibody

Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. DERL1 may act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. It may mediate the interaction between VCP and the degradation substrate. In case of infection by cytomegaloviruses, it plays a central role in the export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Also participates in the degradation process of misfolded cytomegalovirus US2 protein.

Product Categories/Family for anti-DERL1 antibody

Signal Transduction; Amyotrophic Lateral Sclerosis (ALS)

References

Oda Y., et.al., J. Cell Biol. 172:383-393(2006). Ye Y., et.al., Proc. Natl. Acad. Sci. U.S.A. 102:14132-14138(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 197927278
• NCBI GeneID: 79139
• NCBI Accession #: NP_001128143.1; NP_077271.1
• NCBI GenBank Nucleotide #: NM_001134671.2
• UniProt Accession #: Q9BUN8; B3KW41; E9PH19
• Molecular Weight: 28801
• NCBI Official Full Name: derlin-1 isoform b
• NCBI Official Synonym Full Names: derlin 1
• NCBI Official Symbol: DERL1
• NCBI Official Synonym Symbols: DER1; DER-1
• NCBI Protein Information: derlin-1
• UniProt Protein Name: Derlin-1
• Protein Family: Probable derlin
• UniProt Gene Name: DERL1
• UniProt Synonym Gene Names: DER1; DERtrin-1
• UniProt Entry Name: DERL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the derlin family. Members of this family participate in the ER-associated degradation response and retrotranslocate misfolded or unfolded proteins from the ER lumen to the cytosol for proteasomal degradation. This protein recognizes substrate in the ER and works in a complex to retrotranslocate it across the ER membrane into the cytosol. This protein may select cystic fibrosis transmembrane conductance regulator protein (CFTR) for degradation as well as unfolded proteins in Alzheimer's disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

DERL1: Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the degradation substrate. In case of infection by cytomegaloviruses, it plays a central role in the export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Also participates in the degradation process of misfolded cytomegalovirus US2 protein. Forms homo- and heterooligomers with DERL2 and DERL3; binding to DERL3 is poorer than that between DERL2 and DERL3. Interacts with AMFR, VIMP/SELS, SEL1L, SYVN1 and VCP, as well as with SEL1L-SYVN1 and VCP-VIMP protein complexes; this interaction is weaker than that observed between DERL2 and these complexes. Interacts with the cytomegalovirus US11 protein. Interacts with NGLY1 and YOD1. Does not bind to EDEM1. Interacts with RNF103. Up-regulated in response to endoplasmic reticulum stress via the ERN1-XBP1 pathway of the unfolded protein response (UPR). Ubiquitous. Belongs to the derlin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Chaperone; Membrane protein, multi-pass; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: membrane; endoplasmic reticulum; early endosome; late endosome; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; protease binding; MHC class I protein binding; receptor activity

Biological Process: retrograde protein transport, ER to cytosol; intracellular transport of viral proteins in host cell; ER-associated protein catabolic process; establishment of protein localization; protein destabilization; unfolded protein response; response to unfolded protein