DENND1B sirna

DENND1B siRNA (Human)

Cat. Num. AAA826962

• Gene Names: DENND1B; FAM31B; C1ORF18; C1orf218
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DENND1B; DENND1B siRNA (Human); C1orf218; FAM31B; DENN domain-containing protein 1B; Connecdenn 2; Protein FAM31B; DENND1B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DENND1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 775

• Applicable Applications for DENND1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DENND1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DENND1B sirna

siRNA to inhibit DENND1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 304571965
• NCBI GeneID: 163486
• NCBI Accession #: NP_001182144.1
• NCBI GenBank Nucleotide #: NM_001195215.1
• UniProt Accession #: Q6P3S1; Q5T3B8; Q5T3B9; Q5T3C1; Q5TAI8; Q6B0I8; Q8NDT1; Q8TBE6; Q9H774; Q9NXU2; B5MD89; D3PFD5
• Molecular Weight: 86,552 Da
• NCBI Official Full Name: DENN domain-containing protein 1B isoform 3
• NCBI Official Synonym Full Names: DENN/MADD domain containing 1B
• NCBI Official Symbol: DENND1B
• NCBI Official Synonym Symbols: FAM31B; C1ORF18; C1orf218
• NCBI Protein Information: DENN domain-containing protein 1B
• UniProt Protein Name: DENN domain-containing protein 1B
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: DENND1B
• UniProt Synonym Gene Names: C1orf218; FAM31B
• UniProt Entry Name: DEN1B_HUMAN

NCBI Description

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Uniprot Description

DENND1B: a guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Interacts with RAB35. Interacts with clathrin heavy chain/CLTC and with AP2A2. Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells. Five isoforms of the human protein are produced by alternative splicing. Isoform 5 interacts with AP2B1.

Protein type: GEFs; GEFs, Rab

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: clathrin-coated vesicle; cytosol

Molecular Function: Rab guanyl-nucleotide exchange factor activity

Biological Process: protein transport