DAND5 sirna

DAND5 siRNA (Human)

Cat. Num. AAA8222531

• Gene Names: DAND5; SP1; CER2; COCO; CRL2; CERL2; DANTE; GREM3; CKTSF1B3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DAND5; DAND5 siRNA (Human); CER2; CKTSF1B3; GREM3; SP1; DAN domain family member 5; Cerberus-like protein 2; Cerl-2; Cysteine knot superfamily 1. BMP antagonist 3; Gremlin-3; DAND5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DAND5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 189

• Applicable Applications for DAND5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DAND5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DAND5 sirna

siRNA to inhibit DAND5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22749329
• NCBI GeneID: 199699
• NCBI Accession #: NP_689867.1
• NCBI GenBank Nucleotide #: NM_152654.2
• UniProt Accession #: Q8N907
• Molecular Weight: 20,180 Da
• NCBI Official Full Name: DAN domain family member 5
• NCBI Official Synonym Full Names: DAN domain family member 5, BMP antagonist
• NCBI Official Symbol: DAND5
• NCBI Official Synonym Symbols: SP1; CER2; COCO; CRL2; CERL2; DANTE; GREM3; CKTSF1B3
• NCBI Protein Information: DAN domain family member 5
• UniProt Protein Name: DAN domain family member 5
• Protein Family: DAN domain family
• UniProt Gene Name: DAND5
• UniProt Synonym Gene Names: CER2; CKTSF1B3; GREM3; SP1; Cerl-2
• UniProt Entry Name: DAND5_HUMAN

NCBI Description

This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry. [provided by RefSeq, Jul 2008]

Uniprot Description

DAND5: Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, tissue growth and differentiation. Belongs to the DAN family.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular region

Molecular Function: morphogen activity

Biological Process: negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of BMP signaling pathway