DACT1 sirna

DACT1 siRNA (Human)

Cat. Num. AAA8235131

• Gene Names: DACT1; DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DACT1; DACT1 siRNA (Human); DPR1; HNG3; Dapper homolog 1; hDPR1; Dapper antagonist of catenin 1; Hepatocellular carcinoma novel gene 3 protein; DACT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DACT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 799

• Applicable Applications for DACT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DACT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DACT1 sirna

siRNA to inhibit DACT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 118498364
• NCBI GeneID: 51339
• NCBI Accession #: NP_001072988.1
• NCBI GenBank Nucleotide #: NM_001079520.1
• UniProt Accession #: Q9NYF0; Q86TY0; A8MYJ2
• Molecular Weight: 86,212 Da
• NCBI Official Full Name: dapper homolog 1 isoform 2
• NCBI Official Synonym Full Names: dishevelled-binding antagonist of beta-catenin 1
• NCBI Official Symbol: DACT1
• NCBI Official Synonym Symbols: DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
• NCBI Protein Information: dapper homolog 1
• UniProt Protein Name: Dapper homolog 1
• Protein Family: Dapper
• UniProt Gene Name: DACT1
• UniProt Synonym Gene Names: DPR1; HNG3; hDPR1
• UniProt Entry Name: DACT1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, and positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord and head structures, and mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

DACT1: Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2 and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. Interacts with DVL1 and DVL2. Belongs to the dapper family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; cytoplasm; synapse; beta-catenin destruction complex; cytosol; cell junction

Molecular Function: protein binding; protein kinase C binding; protein kinase A binding; beta-catenin binding

Biological Process: negative regulation of JNK cascade; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; dendrite morphogenesis; positive regulation of protein catabolic process; synapse organization and biogenesis; regulation of protein stability; positive regulation of fat cell differentiation; gastrulation with mouth forming second; negative regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; embryonic hindgut morphogenesis