CSPG4 sirna

CSPG4 siRNA (Human)

Cat. Num. AAA8218416

• Gene Names: CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CSPG4; CSPG4 siRNA (Human); MCSP; Chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; CSPG4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CSPG4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2322

• Applicable Applications for CSPG4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CSPG4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CSPG4 sirna

siRNA to inhibit CSPG4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 47419930
• NCBI GeneID: 1464
• NCBI Accession #: NP_001888.2
• NCBI GenBank Nucleotide #: NM_001897.4
• UniProt Accession #: Q6UVK1; Q92675; D3DW77
• Molecular Weight: 250,537 Da
• NCBI Official Full Name: chondroitin sulfate proteoglycan 4
• NCBI Official Synonym Full Names: chondroitin sulfate proteoglycan 4
• NCBI Official Symbol: CSPG4
• NCBI Official Synonym Symbols: NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
• NCBI Protein Information: chondroitin sulfate proteoglycan 4
• UniProt Protein Name: Chondroitin sulfate proteoglycan 4
• Protein Family: Chondroitin sulfate proteoglycan
• UniProt Gene Name: CSPG4
• UniProt Synonym Gene Names: MCSP
• UniProt Entry Name: CSPG4_HUMAN

NCBI Description

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Uniprot Description

NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: lysosomal lumen; focal adhesion; cell surface; Golgi lumen; integral to plasma membrane; apical plasma membrane; extracellular region

Molecular Function: signal transducer activity; protein kinase binding

Biological Process: chondroitin sulfate metabolic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; activation of MAPK activity; carbohydrate metabolic process; chondroitin sulfate catabolic process; tissue remodeling; pathogenesis; angiogenesis; glial cell migration; dermatan sulfate biosynthetic process; transmembrane receptor protein tyrosine kinase signaling pathway