Human chondroitin sulfate proteoglycan 4 ELISA Kit | CSPG4 elisa kit

Human Chondroitin sulfate proteoglycan 4, CSPG4 ELISA Kit

Cat. Num. AAA937702

• Gene Names: CSPG4; NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
• Reactivity: Human
• Synonyms: chondroitin sulfate proteoglycan 4; Human Chondroitin sulfate proteoglycan 4; CSPG4 ELISA Kit; Human Chondroitin sulfate proteoglycan 4 (CSPG4) ELISA kit; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2; chondroitin sulfate proteoglycan 4 (melanoma-associated) ; melanoma-associated chondroitin sulfate proteoglycan; CSPG4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CSPG4. No significant cross-reactivity or interference between human CSPG4 and analogues was observed.
• Sequence Length: 2322

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 25 pg/ml-1600 pg/ml
• Sensitivity: < 6.25 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CSPG4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CSPG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CSPG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CSPG4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CSPG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 47419930
• NCBI GeneID: 1464
• NCBI Accession #: NP_001888.2
• NCBI GenBank Nucleotide #: NM_001897.4
• UniProt Accession #: Q6UVK1; Q92675; D3DW77
• Molecular Weight: 250,537 Da
• NCBI Official Full Name: chondroitin sulfate proteoglycan 4
• NCBI Official Synonym Full Names: chondroitin sulfate proteoglycan 4
• NCBI Official Symbol: CSPG4
• NCBI Official Synonym Symbols: NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
• NCBI Protein Information: chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan NG2; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; chondroitin sulfate proteoglycan 4 (melanoma-associated)
• UniProt Protein Name: Chondroitin sulfate proteoglycan 4
• Protein Family: Chondroitin sulfate proteoglycan
• UniProt Gene Name: CSPG4
• UniProt Synonym Gene Names: MCSP
• UniProt Entry Name: CSPG4_HUMAN

NCBI Description

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]

Uniprot Description

NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: lysosomal lumen; focal adhesion; cell surface; Golgi lumen; integral to plasma membrane; apical plasma membrane; extracellular region

Molecular Function: signal transducer activity; protein kinase binding

Biological Process: chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; activation of MAPK activity; tissue remodeling; pathogenesis; glial cell migration; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; carbohydrate metabolic process; chondroitin sulfate catabolic process; angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway