CHST15 sirna

CHST15 siRNA (Human)

Cat. Num. AAA828502

• Gene Names: CHST15; BRAG; GALNAC4S-6ST
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHST15; CHST15 siRNA (Human); BRAG; GALNAC4S6ST; KIAA0598; Carbohydrate sulfotransferase 15; B-cell RAG-associated gene protein; hBRAG; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; GalNAc4S-6ST; CHST15 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CHST15 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 561

• Applicable Applications for CHST15 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHST15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHST15 sirna

siRNA to inhibit CHST15 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 398303854
• NCBI GeneID: 51363
• NCBI Accession #: NP_001257693.1
• NCBI GenBank Nucleotide #: NM_001270764.1
• UniProt Accession #: Q7LFX5; O60338; O60474; Q86VM4
• Molecular Weight: 58,109 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 15 isoform 1
• NCBI Official Synonym Full Names: carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
• NCBI Official Symbol: CHST15
• NCBI Official Synonym Symbols: BRAG; GALNAC4S-6ST
• NCBI Protein Information: carbohydrate sulfotransferase 15
• UniProt Protein Name: Carbohydrate sulfotransferase 15
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST15
• UniProt Synonym Gene Names: BRAG; GALNAC4S6ST; KIAA0598; hBRAG; GalNAc4S-6ST
• UniProt Entry Name: CHSTF_HUMAN

NCBI Description

Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

CHST15: Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.8.2.33; Glycan Metabolism - chondroitin sulfate biosynthesis; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity; 3'-phosphoadenosine 5'-phosphosulfate binding

Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; hexose biosynthetic process