CHFR sirna

CHFR siRNA (Human)

Cat. Num. AAA8236289

• Gene Names: CHFR; RNF116; RNF196
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHFR; CHFR siRNA (Human); RNF196; E3 ubiquitin-protein ligase CHFR; Checkpoint with forkhead and RING finger domains protein; RING finger protein 196; CHFR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CHFR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 572

• Applicable Applications for CHFR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHFR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHFR sirna

siRNA to inhibit CHFR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239048792
• NCBI GeneID: 55743
• NCBI Accession #: NP_001154816.1
• NCBI GenBank Nucleotide #: NM_001161344.1
• UniProt Accession #: Q96EP1; Q96SL3; Q9NRT4; Q9NT32; Q9NVD5; A6NEN5; B4DZ77; B4E2L6
• Molecular Weight: 63,873 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CHFR isoform 1
• NCBI Official Synonym Full Names: checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
• NCBI Official Symbol: CHFR
• NCBI Official Synonym Symbols: RNF116; RNF196
• NCBI Protein Information: E3 ubiquitin-protein ligase CHFR
• UniProt Protein Name: E3 ubiquitin-protein ligase CHFR
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: CHFR
• UniProt Synonym Gene Names: RNF196
• UniProt Entry Name: CHFR_HUMAN

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]

Uniprot Description

CHFR: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML). Ubiquitous. Belongs to the CHFR family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: PML body; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; nucleotide binding; ligase activity

Biological Process: modification-dependent protein catabolic process; ubiquitin-dependent protein catabolic process; mitosis; protein polyubiquitination; cell division; mitotic cell cycle checkpoint