CELSR3 sirna

CELSR3 siRNA (Human)

Cat. Num. AAA8240103

• Gene Names: CELSR3; FMI1; EGFL1; HFMI1; MEGF2; ADGRC3; CDHF11; RESDA1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CELSR3; CELSR3 siRNA (Human); CDHF11; EGFL1; FMI1; KIAA0812; MEGF2; Cadherin EGF LAG seven-pass G-type receptor 3; Cadherin family member 11; Epidermal growth factor-like protein 1; EGF-like protein 1; Flamingo homolog 1; hFmi1; Multiple epidermal growth factor-like domains protein 2; Multiple EGF-like domains protein 2; CELSR3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CELSR3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3312

• Applicable Applications for CELSR3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CELSR3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CELSR3 sirna

siRNA to inhibit CELSR3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 145309304
• NCBI GeneID: 1951
• NCBI Accession #: NP_001398.2
• NCBI GenBank Nucleotide #: NM_001407.2
• UniProt Accession #: Q9NYQ7; O75092
• Molecular Weight: 358,640 Da
• NCBI Official Full Name: cadherin EGF LAG seven-pass G-type receptor 3
• NCBI Official Synonym Full Names: cadherin, EGF LAG seven-pass G-type receptor 3
• NCBI Official Symbol: CELSR3
• NCBI Official Synonym Symbols: FMI1; EGFL1; HFMI1; MEGF2; ADGRC3; CDHF11; RESDA1
• NCBI Protein Information: cadherin EGF LAG seven-pass G-type receptor 3
• UniProt Protein Name: Cadherin EGF LAG seven-pass G-type receptor 3
• Protein Family: Cadherin EGF LAG seven-pass G-type receptor
• UniProt Gene Name: CELSR3
• UniProt Synonym Gene Names: CDHF11; EGFL1; FMI1; KIAA0812; MEGF2; EGF-like protein 1; hFmi1; Multiple EGF-like domains protein 2
• UniProt Entry Name: CELR3_HUMAN

NCBI Description

This gene belongs to the flamingo subfamily, which is included in the cadherin superfamily. The flamingo cadherins consist of nonclassic-type cadherins that do not interact with catenins. They are plasma membrane proteins containing seven epidermal growth factor-like repeats, nine cadherin domains and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic feature of their subfamily. The encoded protein may be involved in the regulation of contact-dependent neurite growth and may play a role in tumor formation. [provided by RefSeq, Jun 2013]

Uniprot Description

CELSR3: Receptor that may have an important role in cell/cell signaling during nervous system formation. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; protein binding; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; regulation of protein localization; axonal fasciculation; neuron migration; cilium biogenesis; homophilic cell adhesion