Rabbit SLP-76 Polyclonal Antibody | anti-SLP-76 antibody

SLP-76 Polyclonal Antibody

Cat. Num. AAA3006221

• Gene Names: LCP2; SLP76; SLP-76
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SLP-76; Polyclonal Antibody; SLP-76 Polyclonal Antibody; Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76; LCP2; anti-SLP-76 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SLP-76 polyclonal antibody detects endogenous levels of SLP-76 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 533

• Applicable Applications for anti-SLP-76 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 339-384 of Human SLP-76.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SLP-76 pAb at 1:1000 dilutionLane1:Raw264. 7 whole cell lysate (40ug) Lane2:The Thymus tissue lysate of Mouse (40ug) Lane3:The Thymus tissue lysate of Rat (40ug) Lane4:K562 whole cell lysate (40ug))

Related Product Information for anti-SLP-76 antibody

Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production. When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response. Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1. The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation.

NCBI and Uniprot Product Information

• NCBI GI #: 5031855
• NCBI GeneID: 3937
• NCBI Accession #: NP_005556.1
• NCBI GenBank Nucleotide #: NP_005556.1
• UniProt Accession #: Q13094
• Molecular Weight: ~ 75kDa
• NCBI Official Full Name: lymphocyte cytosolic protein 2
• NCBI Official Synonym Full Names: lymphocyte cytosolic protein 2
• NCBI Official Symbol: LCP2
• NCBI Official Synonym Symbols: SLP76; SLP-76
• NCBI Protein Information: lymphocyte cytosolic protein 2
• UniProt Protein Name: Lymphocyte cytosolic protein 2
• UniProt Gene Name: LCP2
• UniProt Synonym Gene Names: SLP76
• UniProt Entry Name: LCP2_HUMAN

NCBI Description

This gene encodes an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. The encoded protein associates with growth factor receptor bound protein 2, and is thought to play a role TCR-mediated intracellular signal transduction. A similar protein in mouse plays a role in normal T-cell development and activation. Mice lacking this gene show subcutaneous and intraperitoneal fetal hemorrhaging, dysfunctional platelets and impaired viability. [provided by RefSeq, Nov 2016]